Accuracy of depression score: a positive HADS score may only be the tip of the iceberg

Abstract Osteoporosis is a common bone and metabolic disease that characterized by density loss microstructural degeneration. Human marrow-derived mesenchymal stem cells (hMSCs) are multipotent progenitor with the potential to differentiate into various cell types, including osteoblasts, chondrocytes, adipocytes, which have been utilized extensively in field of tissue engineering cell-based therapy. Although fluid shear stress plays an important role osteogenic differentiation, cellular molecular mechanisms underlying this effect remain poorly understood. Here, LNA/DNA nanobiosensor was exploited monitor mRNA gene expression hMSCs were exposed physiologically relevant examine regulatory Notch signaling during differentiation. First, effects on viability, proliferation, morphology, differentiation investigated compared. Our results showed modulates morphology depending applied duration. By incorporating alkaline phosphatase (ALP) staining, we further regulating Pharmacological treatment disrupt investigate govern induced experimental provide convincing evidence supporting regulates through signaling. Inhibition mediates reduced ALP enzyme activity decreased Dll4 expression. In conclusion, our will add new information concerning under Further studies may elucidate mechanosensitive